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Exercise 1: Cell Transport Mechanisms and Permeability: Activity 5: Simulating Active Transport Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
- The movement of sodium and potassium maintained by the Na+ -K+ pump
You correctly answered: e. requires energy and is against a concentration gradient. - The sodium-potassium pump is classified as a(n)
You correctly answered: a. antiporter. - The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously.
You correctly answered: b. 3, 2 - Solutes that require active transport for movement might be too large to pass or might be
You correctly answered: a. lipid insoluble.
Experiment Results
Predict Question:
Predict Question 1: What do you think will result from these experimental conditions?
Your answer : c. No Na+ will be transported.
Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium?
Your answer : d. No, it will not affect the transport of either ion.
Stop & Think Questions:
Why did the sodium transport stop before the transport was completed?
You correctly answered: c. The ATP was depleted.
Why was the equilibrium for the solutes reached earlier?
You correctly answered: c. There were more pumps for transport.
Experiment Data:
Run Number Solute ATP Start Conc. L Start Conc.
R
Pumps Carriers Rate
1 Na+ Cl- 1.00 9.00 0.00 500 --- 0.0188
1 K+ Cl- 1.00 0.00 6.00 500 --- 0.0125
2 Na+ Cl- 3.00 9.00 0.00 500 --- 0.0050
2 K+ Cl- 3.00 0.00 6.00 500 --- 0.0033
3 Na+ Cl- 3.00 9.00 0.00 500 --- 0.0000
4 Na+ Cl- 3.00 9.00 0.00 800 --- 0.0083
4 K+ Cl- 3.00 0.00 6.00 800 --- 0.0056
5 Na+ Cl- 3.00 9.00 6.00 800 --- 0.0083
5 K+ Cl- 3.00 0.00 6.00 800 --- 0.0056
5 Glucose --- 0.00 10.00 --- 400 0.0046#
Predict Question:
Predict Question 1: What do you think will result from these experimental conditions?
Your answer : c. No Na+ will be transported.
Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium?
Your answer : d. No, it will not affect the transport of either ion.
Stop & Think Questions:
Why did the sodium transport stop before the transport was completed?
You correctly answered: c. The ATP was depleted.
Why was the equilibrium for the solutes reached earlier?
You correctly answered: c. There were more pumps for transport.
Experiment Data:
Run Number Solute ATP Start Conc. L Start Conc.
R
Pumps Carriers Rate
1 Na+ Cl- 1.00 9.00 0.00 500 --- 0.0188
1 K+ Cl- 1.00 0.00 6.00 500 --- 0.0125
2 Na+ Cl- 3.00 9.00 0.00 500 --- 0.0050
2 K+ Cl- 3.00 0.00 6.00 500 --- 0.0033
3 Na+ Cl- 3.00 9.00 0.00 500 --- 0.0000
4 Na+ Cl- 3.00 9.00 0.00 800 --- 0.0083
4 K+ Cl- 3.00 0.00 6.00 800 --- 0.0056
5 Na+ Cl- 3.00 9.00 6.00 800 --- 0.0083
5 K+ Cl- 3.00 0.00 6.00 800 --- 0.0056
5 Glucose --- 0.00 10.00 --- 400 0.0046#
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
You scored 100% by answering 4 out of 4 questions correctly.
- What happened when you increased the amount of ATP dispensed with the same concentration of sodium and
potassium on either side of the membrane?
You correctly answered: b. More ions were transported. - At what concentration of ATP were the sodium and potassium maximally transported?
You correctly answered: d. 3 mM ATP - What was the effect of adding more Na+ -K+ pumps to the simulated cell?
You correctly answered: a. Transport of the ions was faster. - Describe the effect of adding glucose carriers to the sodium and potassium transport.
You correctly answered: c. There was no change in the transport rate because glucose is transported independently.
Review Sheet Results
- Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell,
instead of other concentration ratios.
Your answer:
The Na+/K+ pump works with 3:2 ratio of soudium - potassium. - Explain why there was no sodium transport even though ATP was present. How well did the results compare with your
prediction?
Your answer:
In order to observe working of Na+/K+ pump sodium and potassium need to be present. So sodium alone can not make
pump work. - Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results
compare with your prediction?
Your answer:
Addition of Glucose and its carriers do not help or hinder Na+/K+ pumping. Because glucose transport works independently. - Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your
answer.
Your answer:
It may transported by facilitated diffusion. Cause glucose is a lipid insoluble and too large to pass through the cell
membrane, it requires a glucose carrier.
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