[PhysioEX Chapter 1 exercise 3] PEX-01-03

Solved by ramonistry

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. Which of the following is true of osmosis?
   You correctly answered: c. It is a type of diffusion.
2. Which of the following occurs when a hypertonic solution is added to cells?
   You correctly answered: d. The cells shrink.
3. The variable that affects osmotic pressure is
   You correctly answered: a. the concentration of nondiffusing solutes.
4. The net movement of water would be into the cell in a
   You correctly answered: b. hypotonic solution.

Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have?
Your answer : a. increased pressure
Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
Your answer : c. pressure above the left beaker
Stop & Think Questions:
Why do you think there is no pressure change?
You correctly answered: b. Sodium is able to diffuse through the pores.
Why do you think there was no pressure change?
You correctly answered: c. There is no net movement of water because the solute concentrations are the same.
Experiment Data:
Run Number Solute MWCO Start Conc. L Pres. L Start Conc. R Pres. R Rate
1 Na+ Cl- 20 5.00 170 0.00 0 0.0000
2 Na+ Cl- 20 10.00 340 0.00 0 0.0000
3 Na+ Cl- 50 10.00 0 0.00 0 0.0167
4 Glucose 100 8.00 136 0.00 0 0.0000
5 Glucose 100 8.00 0 8.00 0 0.0000
6 Glucose 200 8.00 0 0.00 0 0.0036
7 Albumin 200 9.00 153 0.00 0 0.0000
Glucose 200 0.00 153 10.00 0 0.0044

Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
   You correctly answered: c. double.
2. Which membrane did not allow Na+ Cl- to pass through?
   You correctly answered: a. 20 MWCO
3. When a solute is able to diffuse through a membrane
   You correctly answered: b. equilibrium is reached.
4. Water diffuses
   You correctly answered: c. toward solutes.

Review Sheet Results

1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well
   did the results compare with your prediction?
   Your answer:
   Increasing the Na+Cl- will increase the osmotic pressure. Cause the more solute added the more water need to pass
   through.
2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
   Your answer:
   Both of diffusion are passive transport of high concentration to low concentration. Osmosis differs from simple diffusion
   cause osmosis diffusion is the diffusion of water through a selective permeable membrane.
3. Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.”
   Your answer:
   It means that as a solute increases and the number of milliosmoles rise, the water will decrease. Because water moves
   toward high concentration of solute.
4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO
   membrane in place. Explain the results. How well did the results compare with your prediction?
   Your answer:
   Glucose diffuses from right beaker to the left beaker until equilibrium is reached. Albumin can't diffuse through the
   membrane cause of its size. So osmotic pressure of 153mmHg as a result. Prediction was right.